In Vitro Study of 532 nm Wavelength Laser Irradiation on Packed Erythrocytes Using Uv-Vis Spectroscopy

Low-level laser wavelengths were used in a variant of medical applications on account of blood rheology modulation ability and microcirculation improvement. Human blood response to low-level laser irradiation (LLLI) provides valuable information regarding reaction of laser light with tissues. The research was designed to elucidate whether in vitro irradiation changes packed red blood cells and whole blood. Blood samples were collected from Wellness Center, Universiti Sains Malaysia. Samples were each split into two equal aliquots recognized as control (non-irradiated) and irradiated groups. Irradiated sample was exposed to LLLI output powers of 60, 80, and 100 mW at wavelength 532 nm with various irradiation time ranged from 30, 60, 90, 120, and 150 s. The findings showed substantial difference in light absorption with different irradiation exposure duration.  The absorption spectrum for packed red blood cells (RBCs) for the irradiated samples before mixed with plasma in return is lower than irradiated samples after mixed with plasma. When compared the absorption spectrum between RBCs is higher than whole blood. Blood samples smeared showed variations in the packed RBCs morphology between the control samples. Blood samples smeared showed no changes in the red blood cell morphology between the control and irradiated samples. The optimum laser dose obtained from the result for RBC packed cell is at 100 mW and 150 s while for whole blood is at 80 mW and 60s exposure time which suggested……..

 Keywords: Low level laser; Blood; UV-vis spectroscopy, Irradiation