NORDIHIDROGUAIARETIC ACID AS BIOPESTICIDE EXTRACTED OF LEAVES AND STEMS OF Larrea tridentada IN TWO LOCALITIES.
Nordihydroguaiaretic acid (NDGA) is a natural phenolic lignin of Larrea tridentata with antifungal activity; this compound also delays the senescence of the fruits to provide further quality and postharvest duration. The NDGA concentration depends on the part of the plant where it is extracted. This study aimed to determine the concentration of NDGA in leaves and seeds of Larrea tridentata from two localities using three solvents for its extraction. The localities were: Mapimí Valley, Durango and La Sauceda, Ramos Arizpe, Coahuila; the extraction solvents used were: 10% vinegar, 10% methanol, and 10% ethanol in water. NDGA extraction was performed following a 2×2×3 factorial arrangement with four completely randomized repeats. HPLC performed NDGA quantification. No significant difference was found in the extraction solvent and vegetative parts; however, in the localities, the highest value was for Sauceda, with 224.85 mg kg-1, compared to Mapimí, with 123.83 mg kg-1 of NDGA. In the interaction of extraction methods and vegetative part, no significant difference was found; the interaction of extraction methods and localities was found a significant difference with ethanol in Sauceda with 233.57 mg kg-1 as the best, the interaction of vegetative part and localities with significance in leaf in the Sauceda with 231.70 mg kg-1, the interaction of extraction methods, vegetative part and localities was significant in leaf with vinegar in La Sauceda with 245.83 mg kg-1, these being the best responses. Vinegar and the leaves of Larrea tridentata from La Sauceda were the best options for elaborating a biorational product.
Keywords: concentration, antifungal, biorational
