An Establishment of the Manufacturing Process, Quality Control of High Pure Herbal Ingredient of Punicalagins (98%) from Pomegranate and the Functional Properties
Punicalagins are the principal ellagitannins present in pomegranate (Punica granatum L.) peel and are recognized for their exceptional antioxidant, anti-inflammatory, antimicrobial, cardioprotective, hepatoprotective, and anticancer properties. Due to their high biological activity, standardized pomegranate extracts enriched to 98% punicalagins have gained significant attention in nutraceutical, pharmaceutical, and functional food industries. The present study describes an industrial-scale manufacturing process for the production of high-purity punicalagin extract from pomegranate peel. The process involves raw material selection, washing, controlled drying, pulverization, hydroalcoholic extraction, filtration, vacuum concentration, resin-based purification, advanced chromatographic purification, and crystallization. Critical process parameters such as extraction solvent composition, temperature, extraction time, purification techniques, and crystallization conditions were optimized to maximize punicalagin recovery while preserving compound stability. The use of adsorption resins and chromatographic techniques enabled the production of a highly purified extract containing up to 98% punicalagins. The developed manufacturing protocol provides a scalable, reproducible, and economically viable approach for producing pharmaceutical-grade punicalagin extract with consistent quality and enhanced bioactive potential. This study highlights the importance of process optimization and quality control in the commercial production of standardized pomegranate-derived bioactive compounds.
Keywords: Punica granatum, Punicalagins, Pomegranate Peel Extract, Ellagitannins, Hydroalcoholic Extraction, Resin Purification, Chromatography, Crystallization, Industrial Manufacturing, Nutraceuticals.
