METAGENOMIC ANALYSIS OF POULTRY GUT MICROBIOTA OF DIALLEL CROSSES BETWEEN NOILER AND NIGERIAN HEAVY ECOTYPE CHICKENS
A novel, unbiased approach of next generation sequencing – Roche 454 GS – FLX technology was used to study the metagenomic analysis of poultry gut microbes. The metagenomic analysis of the cecal microbial genome in 4 genotype chickens (NxN, HxH, NxH and HxN) of the offsprings were carried out. Birds from the 4 genotypes were sacrificed and the cecum collected and stored in EDTA for laboratory analysis. The composition and function of these Nigerian’s poultry cecal microbiota were analyzed using high-through put sequencing of 16SrRNA genes (V1-V9) hyper variable regions. The cecal microbiota of four Nigerian indigenous chicken genotypes were described and compared. The taxonomic composition of the cecal microbiota was determined by blasting the unigenes to the NCBI Ref Sequence protein database. The results showed that the relative abundance of the dominant genera in the cecal of the 4 genotypes were mainly Bacteroides, Prevotella, Allistrips, Lactobacillus and Rikenellaceal, while the relative abundance of the dominant organisms in the phyla are: Bacteroides, Firmicutes, and Actinobacteria. Microbiological diversity was found in the community, with Noiler chicken having the most eveness, followed by main cross (Noiler x Ecotype Chicken), Heavy Ecotype x Heavy Ecotype while the reciprocal cross (Heavy Ecotype x Noiler Chicken) had the least figure. There were a lot of similarities between the four genotypes where it come to genetic diversity between them and composition. More understanding is needed on the Gut microbiota of the poultry birds.
Keys: Microbiota, Metagenomic, Noiler and Heavy Ecotype Chicken.
